RESEARCH STRATEGY
Biochemical Techniques
Protein Purification
Heterologous expression of recombinant protein
Liquid chromatography
• Ion exchange chromatography
• Size exclusion chromatography
• Affinity chromatography
Protein Chemistry
Protein separation by 2-D gel electrophoresis
In-gel trypsin digestion and protein Identification by mass spectrometry
Protein crosslinking
Polyacrylamide gel electrophoresis
Limited proteolysis
Pulse proteolysis
Immunochemistry
Western blotting
Far western blotting
Immuno-precipitation
ELISA
TECHNIQUES
Biophysical Techniques
Analysis of thermodynamic stability of proteins
Kinetic analysis of protein folding and unfolding
Thermodynamic analysis of complex formation
Isothermal titration calorimetry (ITC)
Spectroscopy
• UV spectroscopy
• NMR spectroscopy
• CD spectroscopy
• Fluorescence spectroscopy
Mass spectrometry
MALDI-TOF-TOF mass spectrometry
Molecular Biology Techniques
Site-directed mutagenesis
Ligation: Cohesive-ended, Blunt-ended, three way ligation
Restriction enzyme screening
Polymerase chain reaction
Electrophoresis of nucleic acids
Heat shock transformation
Electroporation
Competent cell preparation
